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I3LUNG: Clinical Validation of a Multimodal AI Tool to Support Immunotherapy Decisions in NSCLC

January 22, 2026medrxiv logopreprint

Authors

Prelaj, A.,Miskovic, V.,Sacco, M.,Ferrarin, A.,Licciardello, C.,Provenzano, L.,Favali, M.,Lerma, L.,Zec, A.,Spagnoletti, A.,Ganzinelli, M.,Lorenzini, D.,Guirges, B.,Invernizzi, L.,Silvestri, C.,Mazzeo, L.,Meazza Prina, M.,Corrao, G.,Ruggirello, M.,Dumitrascu, A. D.,Di Mauro, R. M.,Monzani, D.,Pravettoni, G.,Zanitti, M.,Macocchi, D.,Marino, M.,Cavalli, C.,Romano, R.,Giani, C.,Armato, S. G.,Esposito, A.,Bestvina, C.,Spector, M.,Bogot, N. R.,Basheer, R.,Hafzadi, A. L.,Roisman, L.,Watermann, I.,Szewczyk, M.,Olchers, T.,Richter, H.,Blanke-Roeser, C.,Sinisca

Affiliations (1)

  • Fondazione IRCCS Istituto Nazionale dei Tumori di Milano

Abstract

Despite a decade of immunotherapy, treatment selection in non-small cell lung cancer (NSCLC) still relies on subgroup analyses and clinical scores. I3LUNG (NCT05537922) is currently the largest international, real-world, multimodal, artificial intelligence (AI)-based trial, enrolling 2365 patients. We integrated real-world clinical data (RWD), computed tomography (CT) images, digital pathology (DP), and genomics (G) into machine learning early-fusion (MLEF) and deep-learning intermediate-fusion (DLIF) models. MLEF achieved consistent performance across outcomes (AUC{approx}0.74), with improved results in first-line patients (AUC up to 0.82). Multimodal models outperformed RWD in clinical-specific subgroups (AUCs up to 0.86). In the test set, AI models surpassed PD-L1, ECOG PS, NLR, LDH (all with p<0.01) and the LIPI score. The clinical usability study showed that expert and non-expert physicians could improve their prediction with the explainable AI (XAI) tool. The I3LUNG tool emerges as a clinically relevant decision-support system and is currently under prospective validation in >2,000 patients.

Topics

oncology

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