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Automated Delineation of Putative Non-Contrast-Enhancing Tumor in Glioblastoma: Prognostic Insights.

February 25, 2026pubmed logopapers

Authors

Gómez-Mahiques M,Lopez-Mateu C,Gil-Terrón FJ,Montosa-I-Micó V,Svensson SF,Mendoza Mireles EE,Vik-Mo EO,Emblem KE,Balañà-Quintero C,Puig J,Alenda C,Martinez-Saez E,Martínez-Ricarte F,Quirós-Martí M,Quilis-Quesada V,García-Gómez JM,Fuster-Garcia E

Affiliations (11)

  • Instituto Universitario de TecnologĂ­as de la InformaciĂłn y Comunicaciones, Universitat Politècnica de València, Valencia, Spain.
  • Department for Physics and Computational Radiology, Division of Radiology and Nuclear Medicine, Oslo University Hospital, Oslo, Norway.
  • Vilhelm Magnus Laboratory, Department of Neurosurgery, Oslo University Hospital, Oslo, Norway, and Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway.
  • Institut d'InvestigaciĂł Germans Trias i Pujol, Barcelona, Spain.
  • Department of Radiology and IDIBAPS, Hospital ClĂ­nic de Barcelona, Barcelona, Spain.
  • Pathology Department, Alicante Department of Health-General Hospital, ; Department of Pathology and Surgery, Miguel Hernández University; Alicante Institute for Health and Biomedical Research (ISABIAL), Alicante, Spain.
  • Servicio de AnatomĂ­a PatolĂłgica, Hospital Universitari Vall d'Hebron, Barcelona, Spain.
  • Servicio de NeurocirugĂ­a, Hospital Universitari Vall d'Hebron, Barcelona, Spain.
  • Servicio de NeurocirugĂ­a, Hospital ClĂ­nic Universitari de València, València, Spain.
  • Departamento de CirugĂ­a, Facultat de Medicina, Universitat de València, València, Spain.
  • Department of Neurosurgery, Mayo Clinic, Jacksonville, Florida, USA.

Abstract

Precise delineation of non-contrast-enhancing tumor (nCET) in glioblastoma (GB) is critical for maximal safe resection, yet routine imaging cannot reliably separate infiltrative tumor from vasogenic edema. The aim of this study was to develop and validate an automated method to identify peritumoral subregions compatible with nCET and assess its prognostic value. Pre-operative T2-weighted and FLAIR MRI from 940 patients with newly diagnosed GB in four multicenter cohorts were analyzed. A deep-learning model segmented enhancing tumor, edema and necrosis; a non-local, spatially varying finite mixture model was applied to identify edema subregions characterized by relatively lower FLAIR hyperintensity, hypothesized to reflect nCET-related tissue. The ratio of these subregions to total edema volume defined the T2/FLAIR Heterogeneity Index (TFHI). Associations between TFHI and overall survival (OS) were examined with Kaplan-Meier curves and multivariable Cox regression. Higher TFHI values stratified patients with shorter OS. In the NCT03439332, TFHI above the optimal threshold was associated with a twofold increased hazard of death (hazard ratio (HR) 2.07, 95 % confidence interval 1.33-3.21; p = 0.0013) and a reduction in median survival of 98 days. Significant, though smaller, prognostic effects were confirmed in GLIOCAT & BraTS (HR= 1.37; p = 0.047), OUS (HR = 1.37; p = 0.0032) and pooled analysis (HR= 1.26; p = 0.0008). TFHI remained an independent predictor after adjustment for age, extent of resection and MGMT methylation. We present a reproducible, server-hosted tool for automated identification of imaging-defined, putative nCET-related peritumoral subregions and TFHI biomarker extraction that enables independent prognostic stratification. This approach provides a quantitative framework for studying peritumoral heterogeneity in GB.

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Journal Article

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